Flow cytometric detection of dyserythropoiesis: a sensitive and powerful diagnostic tool for myelodysplastic syndromes. | Aplastic Anemia and MDS International Foundation

Flow cytometric detection of dyserythropoiesis: a sensitive and powerful diagnostic tool for myelodysplastic syndromes.

Journal Title: 
Leukemia
Author(s): 
Mathis S, Chapuis N, Debord C, Rouquette A, Radford-Weiss I, Park S, Dreyfus F, Lacombe C, Béné MC, Kosmider O, Fontenay M, Bardet V.
Primary Author: 
Mathis S
Original Publication Date: 
Friday, June 14, 2013

Several groupshave published flow cytometry scores useful for the diagnosis or prognosis of myelodysplastic syndromes (MDS), mainly based on the detection of immunophenotypic abnormalities in the maturation of granulocytic/monocytic and lymphoid lineages. Because anemia is the most frequent symptom of early MDS, the aim of this study was to identify markers of dyserythropoiesis relevant for the diagnosis of MDS, analyzed by selecting erythroblasts in a whole no-lysis bone marrow strategy by using a nuclear dye. This prospective study included 163 patients, including 126 with cytopenias leading to MDS suspicion and 46 controls without MDS. In a 'learning cohort' of 53 unequivocal MDS with specific markers, there was a significant difference between the coefficients of variation of mean fluorescence intensities of CD71 and CD36 in MDS patients compared to controls. These two parameters and the haemoglobin level were used to build a 'RED-score' strongly suggestive of MDS if≥3. Using the 'RED-score' in the whole cohort, 80% of MDS or non MDS patients were correctly classified. When combined with the flow-score described by Ogata, this strategy allowed to reach a very high sensitivity of 88% of patients correctly classified.Leukemia accepted article preview online, 14 June 2013; doi:10.1038/leu.2013.178.

Bone Marrow Diseases: